This study investigates age-related changes in mitochondrial 3D structure in human skeletal muscle and shows that exercise can restore mitochondrial integrity through mitofusin 2.

bioRxiv · Nov 13, 2023Biophysics

Cardiovascular Hemodynamics in Mice with Tumor Necrosis Factor Receptor - Associated Factor 2 Mediated Cytoprotection in the Heart

A. G. Marshall, K. Neikirk, Z. Vue, H. K. Beasley, E. Garza-Lopez, L. Vang, T. Barongan, Z. Evans, A. Crabtree, E. Spencer, J. Anudokem, R. Parker, J. Davis, D. Stephens, S. Damo, , A. K. Reddy

This study evaluates global cardiac function in mice with cardiac-restricted TRAF2 overexpression, finding diminished cardiac function despite reported tolerance to ischemic insults.

bioRxiv · Sep 3, 2022Biophysics

Three-Dimensional Mitochondria Reconstructions of Murine Cardiac Muscle Changes in Size Across Aging

Z. Vue, K. Neikirk, L. Vang, E. Garza-Lopez, T. A. Christensen, J.-q. Shao, J. Lam, H. K. Beasley, A. G. Marshall, A. Crabtree, J. Anudokem, B. Rodriguez, B. Kirk, S. Bacevac, T. Barongan, , A. Hinton

This study uses 3D imaging to show that mitochondria in aging mouse hearts lose cristae structure and volume, similar to mutations in MICOS complex genes.

bioRxiv · Apr 23, 2022Biophysics

Systematic Transmission Electron Microscopy-Based Identification and 3D Reconstruction of Cellular Degradation Machinery

K. Neikirk, Z. Vue, P. Katti, B. I. Rodriguez, S. A. Omer, J. Shao, T. A. Christensen, E. Garza Lopez, A. Marshall, C. Palavicino-Maggio, J. Ponce, A. Alghanem, L. Vang, T. Barongan, H. Beasley, , A. Hinton

This paper describes methods to identify and distinguish subcellular structures involved in macroautophagy using TEM and 3D reconstruction, with applications to quantify degradation machinery under different conditions.

bioRxiv · Sep 26, 2021Biophysics