Addressing viral genomic variability towards developing a Cas13b-based therapy
Mai Anh Thu Le, Laura C. McCoullough, Zak T. Janetzki, Yi Wen Liaw, Mohamed Fareh, Joseph A. Trapani, Peter A. Revill, Margaret Littlejohn
This study evaluates how viral genomic variability affects CRISPR-Cas13b efficacy against hepatitis B virus, showing that mismatched guide RNAs reduce antiviral activity and that sequence specificity is not the sole determinant of efficacy.
bioRxiv · Jul 17, 2026 · PreprintMicrobiology