Deep profiling reveals substantial heterogeneity of integration outcomes in CRISPR knock-in experiments
Hera Canaj, Jeffrey A. Hussmann, Han Li, Kyle A. Beckman, Leeanne Goodrich, Nathan H. Cho, Yucheng J. Li, Daniel A. Santos, Aaron McGeever, Edna M. Stewart, Veronica Pessino, Mohammad A. Mandegar, Cindy Tzu-Ling Huang, Li Gan, Barbara Panning, , Manuel D. Leonetti
Long-read sequencing with the knock-knock pipeline profiles CRISPR knock-in repair, finding perfect homology-directed repair is often a minority of integration events and mis-integration varies by context.
bioRxiv · Nov 13, 2019 · PreprintGenomics