Han Li

The paper reprograms the Rossmann fold motif to create NMN+-specific enzymes that avoid NAD(P)/H interactions, achieving high specificity and productivity in biotransformation.

The paper identifies a conserved sequence motif that enables natural enzymes to use non-canonical redox cofactors, demonstrating high activity with NMN+ and other synthetic cofactors.

This paper demonstrates an activation-free method to convert carboxylic acids to aldehydes and alcohols using aldehyde dehydrogenases, achieving high yields and carbon efficiency.

This paper engineers Escherichia coli GapA to use NMN+ instead of NAD+, achieving a high cofactor specificity switch through rational design and mutagenesis.

This paper introduces Nox Ortho, an enzyme that enables precise control of redox reactions using NMN+ as a cofactor, achieving high specificity and enabling chiral-pure product synthesis.

This paper engineers Escherichia coli to biosynthesize nicotinamide mononucleotide (NMN+) for use in cell-free biosynthesis, achieving a 130-fold increase in intracellular NMN+ levels.

Long single-stranded DNA donors enable gene-sized CRISPR knock-in in human cells, though an author update notes subsequent long-read sequencing revealed high imprecise integration rates for both donor types.

Long-read sequencing with the knock-knock pipeline profiles CRISPR knock-in repair, finding perfect homology-directed repair is often a minority of integration events and mis-integration varies by context.

The paper applies local PCA to genomic data from three species, showing population structure effects vary across genomic regions, likely due to linked selection and chromosomal inversions.