Author

Daniel A. Santos

Howard Hughes Medical InstituteORCID 0000-0002-3757-189XUnclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Assay:CRISPR screen· 1 Approach:deep learning· 1 Research Focus:gene regulatory network inference· 1 Organism:human· 1 Assay:long-read sequencing· 1 Research Focus:perturbation response prediction· 1 Assay:scRNA-seq· 1

Frequent Co-Authors

Jeffrey A. HussmanniD· 2 shared Jonathan S. WeissmaniD· 2 shared Aaron McGeeveriD· 1 shared Barbara PanningiD· 1 shared Bo HuangiD· 1 shared Carol A. GrossiD· 1 shared Christina R. LiemiD· 1 shared Cindy Tzu-Ling HuangiD· 1 shared

Activity by Year

2019-2020 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 33 mutual co-author links

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2 shared papersGenomics

Jeffrey A. HussmannORCID

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2 papers

Recent·Most cited

Titrating gene expression with series of systematically compromised CRISPR guide RNAs

Marco Jost, Daniel A. Santos, Reuben A. Saunders, Max A. Horlbeck, John S. Hawkins, Sonia M. Scaria, Thomas M. Norman, Jeffrey A. Hussmann, Christina R. Liem, Carol A. Gross, Jonathan S. Weissman

This paper develops a method to control gene expression using CRISPR interference and deep learning, revealing gene-specific expression-phenotype relationships.

Nature Biotechnology · Jan 13, 2020Peer-reviewedGenomics

Deep profiling reveals substantial heterogeneity of integration outcomes in CRISPR knock-in experiments

Hera Canaj, Jeffrey A. Hussmann, Han Li, Kyle A. Beckman, Leeanne Goodrich, Nathan H. Cho, Yucheng J. Li, Daniel A. Santos, Aaron McGeever, Edna M. Stewart, Veronica Pessino, Mohammad A. Mandegar, Cindy Tzu-Ling Huang, Li Gan, Barbara Panning, , Manuel D. Leonetti

Long-read sequencing with the knock-knock pipeline profiles CRISPR knock-in repair, finding perfect homology-directed repair is often a minority of integration events and mis-integration varies by context.

bioRxiv · Nov 13, 2019 · PreprintGenomics