Rohit Kulkarni

The paper uses single-islet proteomics to analyze pseudo-temporal immune responses and beta-cell dysfunction in stage 1 T1D, identifying key pathways involved in islet dysfunction.

This study compares 20 nm and 35 nm pore size SEC columns for EV isolation, finding that smaller pores improve yield and purity in cell culture media and plasma.

This paper uses top-down proteomics to analyze changes in proteoforms of human pancreatic islets after cytokine treatment, identifying potential markers for T1D progression.

Laser-microdissected islets of 3 multiple-autoantibody-positive stage 1 type 1 diabetes donors versus matched controls show 202 altered proteins: immune and glycolytic up, translation and ER-stress proteins down.

scPrediXcan improves transcriptome-wide association studies by integrating deep learning for cell-type-specific gene expression prediction, identifying more causal genes and explaining more GWAS loci in type 2 diabetes and systemic lupus erythematosus.

This study shows that m6A methylation, regulated by METTL3, controls the innate immune response in β-cells during T1D onset by modulating OAS mRNA stability.

This study investigates the role of m6A methylation in early pancreatic β-cell differentiation in humans and mice, showing METTL14's essential role in this process.

This study shows that fructose metabolism via KHK-C increases ER stress and modulates the hepatic transcriptome, contributing to NAFLD progression in both genetic and diet-induced models.

RADAR is a tool for differential methylation analysis of MeRIP-seq data, showing improved accuracy and reproducibility compared to existing methods.