Amanda McQuade

This study identifies 772 genes regulating the interferon-responsive microglia state using CRISPRi screening in human iPSC-derived microglia, including novel regulators like CNOT10.

The paper identifies regulators of microglial activation states using CRISPR screens and single-cell transcriptomics, revealing functional roles of ZNF532, PRDM1, and DNMT1 in disease-associated microglial states.

The paper identifies strategies to prevent transgene silencing during human pluripotent stem cell differentiation and introduces a robust vector construct.

The paper uses CRISPRi to screen ASD risk genes in microglia, identifying ADNP's role in synaptic pruning and endocytosis.

This study shows that ER and SOCE Ca2+ signals are not required for directed migration of human microglia in response to purinergic stimuli, suggesting a Ca2+-independent mechanism involving cyclic AMP.

This study shows that TREM2 loss in human iPSC-derived microglia leads to altered Ca2+ signaling and motility via purinergic receptor pathways.

This study examines C9orf72 ALS/FTD patient-derived iPSC-microglia mono-cultures, finding they form RNA foci, have reduced C9orf72 protein, and show endosomal-lysosomal dysfunction, but no significant microglial-enriched gene expression differences.