Karen Marie Polizzi

The paper develops a bioorthogonal reporter for MGAT1 by iteratively engineering a UDP-GlcNAc analog with a chemical tag, enabling specific substrate incorporation in mammalian cells.

The paper quantifies resource load from genetic constructs in mammalian cells and provides a framework for designing more efficient synthetic circuits.

The paper introduces CHOmpact, a reduced metabolic model of CHO cells that improves interpretability and physiological consistency compared to large-scale models.

The paper demonstrates a method to generate diverse halogenated and derivatized analogues of the natural product violacein using synthetic biology and chemistry.

The paper develops a curcumin-based DiPro biosensor for cell-free biosynthesis, achieving an 80-fold increase in fluorescence through optimized reaction parameters.

This paper develops a low-cost agglutination assay using E. coli-displayed nanobodies for detecting biomarkers with high selectivity and low detection limits, suitable for point-of-care testing.

The paper develops a library of AHL-receiver devices and a software tool to identify orthogonal chemical communication channels for synthetic microbial consortia.

This work computationally redesigns synthetic genetic oscillators to enable independent amplitude and frequency modulation using tuneable degradation and additional input signals.

This paper describes a cell-free synthetic biochemistry platform that achieves high yield raspberry ketone production through an optimised enzymatic pathway.