Roberto Di Blasi

Degradation Bottlenecks and Resource Competition in Transiently and Stably Engineered Mammalian Cells

This study investigates degradation bottlenecks and resource competition in mammalian cells using degron tags, showing that degradation rates of proteins are coupled due to competition for cellular resources.

Mammalian cell characterisation by non-invasive plate reader assay

This paper develops a non-invasive plate reader assay for high-throughput mammalian cell analysis, showing its applicability to different cell lines and conditions.

Resource-aware construct design in mammalian cells

The paper quantifies resource load from genetic constructs in mammalian cells and provides a framework for designing more efficient synthetic circuits.

Regulatory control circuits for stabilizing long-term anabolic product formation in yeast

The paper designs synthetic control circuits in yeast to stabilize vanillin-β-glucoside production over multiple generations, achieving higher yields in fermentation.