Paul S. Freemont

Hollow-fibre biomanufacturing and cell-free engineering of HEK293 extracellular vesicles

This paper demonstrates cell-free engineering of HEK293 extracellular vesicles with membrane fusion proteins and evaluates methods for assessing engineering efficiency.

Metabolomics-informed coarse-grained model enables prediction of cell-free protein expression dynamics

The paper develops a coarse-grained model of cell-free protein synthesis using metabolomics data and Bayesian inference to predict expression dynamics across novel conditions.

Optimizing protein production in the One-Pot PURE system: insights into reaction composition and expression efficiency

This study optimizes the One-Pot PURE system by addressing expression instability and proteolysis in E. coli strains, improving protein production efficiency.

Biosynthesis of Arcyriaflavin F from Streptomyces venezuelae ATCC 10712

This paper reports a new indolocarbazole biosynthetic pathway in Streptomyces venezuelae and identifies a regioselective C-5 hydroxylating enzyme.

Simple, low-cost production of DNA MS2 virus-like particles as molecular diagnostic controls

The paper describes a simple, low-cost method to produce DNA MS2 virus-like particles as diagnostic controls, validated using droplet digital PCR and WHO standards.

basicsynbio and the BASIC SEVA collection: Software and vectors for an established DNA assembly method

The paper introduces basicsynbio, an open-source software tool for designing and assembling DNA sequences using the BASIC method, and presents the BASIC SEVA collection of vectors.

A GenoChemetic strategy for derivatization of the violacein natural product scaffold

The paper demonstrates a method to generate diverse halogenated and derivatized analogues of the natural product violacein using synthetic biology and chemistry.

A curcumin direct protein (DiPro) biosensor for cell-free prototyping

The paper develops a curcumin-based DiPro biosensor for cell-free biosynthesis, achieving an 80-fold increase in fluorescence through optimized reaction parameters.

AL-PHA beads: bioplastic-based protease biosensors for global health applications

The paper develops AL-PHA beads, bioplastic-based protease biosensors, and demonstrates their ability to detect specific proteases in various samples.

A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology

The paper presents an improved Streptomyces venezuelae cell-free TX-TL system with higher protein yields and reduced batch variation, enabling synthetic biology applications.

A biosynthetic platform for antimalarial drug discovery

This paper demonstrates the use of a bacterial biosynthesis platform to produce and screen violacein derivatives for antimalarial activity against Plasmodium falciparum.

A low-cost biological agglutination assay for medical diagnostic applications

This paper develops a low-cost agglutination assay using E. coli-displayed nanobodies for detecting biomarkers with high selectivity and low detection limits, suitable for point-of-care testing.

Cell-free prototyping strategies for enhancing the sustainable production of polyhydroxyalkanoates bioplastics

This study develops cell-free systems to prototype and optimize polyhydroxyalkanoates biosynthesis, showing whey permeate enhances production and identifies enzymes for metabolite recycling.

A cell-free synthetic biochemistry platform for raspberry ketone production

This paper describes a cell-free synthetic biochemistry platform that achieves high yield raspberry ketone production through an optimised enzymatic pathway.