This paper improves split-HaloTag by engineering cpHalo{Delta} with computational methods to enhance thermostability and activity, reducing assay time and substrate requirements.

Protein Science · Apr 18, 2025Peer-reviewedBiochemistry

Crowdsourced Protein Design: Lessons From the Adaptyv EGFR Binder Competition

Tudor‐Stefan Cotet, Igor Krawczuk, Filippo Stocco, Noelia Ferruz, Anthony Gitter, Yoichi Kurumida, Lucas de Almeida Machado, Francesco Paesani, Cianna N. Calia, Chance A. Challacombe, Nikhil Haas, Ahmad Zaman Qamar, Bruno E. Correia, Martin Pačesa, Lennart Nickel, , Adaptyv Competition Organizers

The paper analyzes a crowdsourced protein design competition, showing participants designed EGFR binders with varying affinities and highlighting challenges in benchmarking and experimental design.

bioRxiv · Apr 17, 2025 · PreprintBioengineering

BindCraft: one-shot design of functional protein binders

Martin Pačesa, Lennart Nickel, Christian Schellhaas, Joseph H. Schmidt, Ekaterina Pyatova, Lucas Kissling, Patrick Barendse, Jagrity Choudhury, Srajan Kapoor, Ana Alcaraz‐Serna, Yehlin Cho, Kourosh H. Ghamary, Laura Vinué, Brahm J. Yachnin, Andrew M. Wollacott, , Bruno E. Correia

Hallucinating binders by backpropagating through AlphaFold2 weights gave, across 12 targets, binders in 10-100% of the designs tested per target, mostly at nanomolar affinity.

bioRxiv · Sep 30, 2024 · PreprintBioinformatics✦ In-depth digest