Bungo Akiyoshi

This study introduces an auxin-inducible degron system in Diplonema papillatum to enable conditional protein depletion and assesses its effects on key cellular processes.

This study shows that the KKT2 CL domain binds histone H3 N-terminus and that methylation of this region disrupts binding, suggesting a mechanism for kinetochore assembly in kinetoplastids.

This study identifies 17 proteins localized to the feeding apparatus and flagellar apparatus in Diplonema papillatum, providing foundational insights into their molecular mechanisms.

This study identifies unique mitotic features in Paradiplonema papillatum using fluorescence microscopy and YFP-tagging, revealing novel structures like metaphase rings and potential kinetochore localization.

The paper performs subcellular proteomics on Paradiplonema papillatum, identifying 4,870 proteins across 22 compartments and revealing carbohydrate metabolism capabilities and peroxisome plasticity.

This paper identifies TAC53 as a novel component of the Tripartite Attachment Complex (TAC) in Trypanosoma brucei, revealing its tubular architecture and role in mitochondrial DNA segregation.

This study determines the structure and function of KKT23, a kinetochore acetyltransferase in trypanosomes, showing it acetylates histone H2A and interacts with KKT22.

This study investigates the kinetochore system in diplonemids, a group of protists, and suggests they may have evolved a previously unknown type of kinetochore.

This study shows that Aurora B and KKT14 form an unconventional regulatory pathway controlling mitotic exit and chromosome segregation in trypanosomes.

This study identifies KKT14-KKT15 in kinetoplastids as divergent orthologs of Bub1/BubR1-Bub3, suggesting a unique mechanism for chromosome segregation fidelity in trypanosomes.