Julius Lukeš

This study maps the subcellular proteome of Euglena longa using SSP to identify 5,909 proteins across 16 compartments, with high-confidence localisations for 2,955 proteins.

The paper identifies a novel sense-to-sense codon reassignment in Acidimicrobiales bacteria where ACA specifies aspartate instead of threonine.

The paper analyzes genomes and transcriptomes of Rhynchopus flagellates to assess endosymbiotic gene transfer (EGT) from holosporacean and chlamydiacean symbionts, finding no evidence of recent EGT and highlighting dynamic HGT patterns.

This study characterizes mitochondrial complex composition in Perkinsus marinus, revealing conserved divergent features shared with apicomplexan parasites.

The paper identifies and characterizes the first RNA viruses in trypanosomes, including a leishmaniavirus and two narnaviruses in Trypanosoma platydactyli, suggesting ecological factors influence viral acquisition.

This study identifies unique mitotic features in Paradiplonema papillatum using fluorescence microscopy and YFP-tagging, revealing novel structures like metaphase rings and potential kinetochore localization.

The paper performs subcellular proteomics on Paradiplonema papillatum, identifying 4,870 proteins across 22 compartments and revealing carbohydrate metabolism capabilities and peroxisome plasticity.

The paper shows that near-cognate tRNAs that promote stop codon readthrough form specific contacts with ribosomal proteins at the decoding site, with the anticodon stem length and structure influencing this interaction.

This paper develops a computational model to predict DNA mechanics from sequence, revealing mechanical selection in diverse eukaryotes and identifying extreme DNA mechanics in nature.

This study investigates the kinetochore system in diplonemids, a group of protists, and suggests they may have evolved a previously unknown type of kinetochore.

This paper identifies and characterizes novel kinetoplast-associated proteins in Trypanosoma brucei, revealing their essential roles in mitochondrial DNA replication, segregation, and cell cycle regulation.

This paper identifies TbNAB70 as a protein essential for kinetoplast DNA segregation in Trypanosoma brucei, showing it binds to kDNA and facilitates its proper division.

This study identifies heme-deficient metabolism and impaired cellular differentiation as evolutionary trade-offs for human infectivity in Trypanosoma brucei gambiense due to HpHbR inactivation.

The paper identifies TbKifX2A and TbPH1 as proteins interacting at the trypanosomal microtubule quartet, showing their localization and functional roles in maintaining flagellar pocket structure.