Eva Rohlová

The paper introduces a method to profile microglia from frozen brain tissue using PU.1 as a marker and Smart-seq3xpress, showing improved transcriptomic data compared to standard protocols.

This study benchmarks small RNA-sequencing methods for miRNA quantification in biofluids, finding no single method outperforms others and highlighting protocol-specific biases.

The paper analyzes RNA-Seq data from young and aged mice after ischemic stroke to identify age-related transcriptional changes and inflammatory responses.