PU.1-driven enrichment enables microglia profiling from frozen brain tissue using the high-throughput Smart-seq3xpress method
Dominika Dostalova, Pavel Abaffy, Eva Rohlová, Ján Kriška, Tomáš Knotek, Jana Turečková, Denisa Kirdajová, Miroslava Anděrová, Lukáš Valihrach
The paper introduces a method to profile microglia from frozen brain tissue using PU.1 as a marker and Smart-seq3xpress, showing improved transcriptomic data compared to standard protocols.
bioRxiv · Aug 11, 2025 · PreprintNeuroscience