Mikael Kubista

Introducing the digital PCR data essentials standard to harmonize data structure for clinical and research use

The paper introduces the Digital PCR Data Essentials Standard (DDES) to standardize data structure for dPCR, improving interoperability and reproducibility across platforms and applications.

Spatiotemporal transcriptomic map of ischemic brain injury

This study maps the spatiotemporal transcriptomic changes in mouse brain tissue after ischemic injury, revealing glial cell responses and their role in neuroinflammation.

Characterization of regeneration initiating cells during Xenopus laevis tail regeneration

This study identifies Regeneration Initiating Cells (RICs) in Xenopus laevis tail regeneration using single cell and spatial transcriptomics, showing their role in initiating regeneration by modifying the extracellular matrix.

Small RNA-sequencing for Analysis of Circulating miRNAs: Benchmark Study

This study benchmarks small RNA-sequencing methods for miRNA quantification in biofluids, finding no single method outperforms others and highlighting protocol-specific biases.

Decoding the transcriptional response to ischemic stroke in young and aged mouse brain

The paper analyzes RNA-Seq data from young and aged mice after ischemic stroke to identify age-related transcriptional changes and inflammatory responses.

Performance comparison of reverse transcriptases for single-cell studies

The paper compares 11 reverse transcriptases for single-cell RNA quantification, identifying two top performers that improve sensitivity and clustering resolution in low RNA concentration experiments.