Rose Fields

DNA O-MAP identifies molecular neighborhoods around specific genomic loci using programmable probes and proteomics.

The paper uses O-MAP to study a cardiac-specific RNA factory around TTN RNA, revealing its chromatin interactions, RNA composition, and role of RBM20 in gene regulation.

The paper identifies 2884 calcium-regulated proteins across human, mouse, and yeast proteomes using thermostability assays and reveals novel calcium engagement in metabolic enzymes and the spliceosome.

This study uses quantitative proteomics to analyze human and mouse gastruloids at four stages, revealing protein dynamics, discordance with transcriptomes, and gene networks linked to developmental defects.

The paper introduces O-MAP, a method for discovering RNA-interacting proteins, transcripts, and genomic loci in situ with high precision and without genetic manipulation.