Sara Bianchi

Combinatorial and Inducible CRISPRa/i Enables Canalized hiPSC Forward Programming and Iterative Refinement via Single-Cell Genomics

The paper introduces CIRI, a CRISPR-based platform for programmable cell fate conversion in hiPSCs, using single-cell genomics to identify regulatory factors for myogenic differentiation.

Opposing CTCF and GATA4 activities set the pace of chromatin topology remodeling during cardiomyogenesis

The paper shows that CTCF and GATA4 have opposing roles in chromatin remodeling during cardiomyogenesis, with CTCF inhibiting and GATA4 promoting topological changes.

Nascent transcript O-MAP reveals the molecular architecture of an RBM20-regulated single-locus subnuclear compartment organized around the TTN RNA

The paper uses O-MAP to study a cardiac-specific RNA factory around TTN RNA, revealing its chromatin interactions, RNA composition, and role of RBM20 in gene regulation.