Jin Zhang

The paper develops sensitive FRET biosensors to visualize CaMKII activity dynamics in living cells and demonstrates their use in cultured and primary cells, including neurons and cardiomyocytes.

The paper introduces AKAR6, a new suite of FRET-based PKA sensors with enhanced sensitivity and selectivity for detecting compartment-specific PKA activity in live cells.

This paper develops highly sensitive chemigenetic FRET-based biosensors for kinase and signaling activity with improved dynamic range and spectral properties.

The paper develops a data-informed model of AMPK signaling using uncertainty quantification and biosensors, showing AMPK integrates inputs over time during exercise.

The paper identifies a druggable EP2 receptor in Schwann cells that encodes PG-mediated persistent inflammatory pain without affecting protective inflammation.

This paper uses Bayesian multimodel inference to improve predictive certainty in systems biology models of intracellular signaling pathways.

The paper investigates how Ca2+ oscillations are decoded by the ERK cascade in pancreatic β-cells using fluorescent biosensors and reveals sequential signal processing along the pathway.

The paper introduces far-red chemigenetic kinase activity biosensors enabling five-color multiplexing, 4D imaging, and super-resolution via STED microscopy.

The paper introduces a sensitive fluorescent biosensor, ExRai-CKAR2, to study subcellular PKC activity and finds differential regulation of PKC isoforms at the ER, lysosomes, and Par complexes.

This paper introduces KINACT, a light-gated tool that converts kinase activity into permanent fluorescent marks for analyzing kinase dynamics and signaling mechanisms.

This study identifies molecular determinants of PKA RIα phase separation and shows how it regulates cAMP compartmentation and PKA-C spatial restriction.

The paper develops computational models of the Ca2+/cAMP pathway and AKAP/AC nanodomains, predicting incoherent feedforward loop behavior in Ca2+/cAMP oscillations.

The paper develops a sensitive AMPK activity reporter, ExRai AMPKAR, to study subcellular AMPK dynamics and reveals differential regulation of AMPK activity at lysosomes, mitochondria, and the nucleus.

The paper identifies genomic regions proximal to nuclear speckles using noncoding RNAs and MARGI assay, showing nsaRNAs interact with specific genomic sites.