Barbara Ciani

This paper develops a 3D expansion microscopy toolkit to visualize and quantify nanoscale interactions in endosomes, revealing EGF and EGFR1 enrichment and Rab5a accumulation during endosomal maturation.

The paper demonstrates that membrane tension, regulated by ILV formation, negatively feedbacks to control ESCRT-III activity in vitro, with potential implications for in vivo functions.

This study shows ESCRT-III and CHMP7 protect against genomic instability in micronuclei by repairing nuclear envelopes but also generate cytosolic DNA that promotes inflammation in cancer cells.