AEGIS identifies epitope- and clone-specific autoimmunity in ROHHAD by linking immune repertoires to self-epitopes, revealing convergence on ZSCAN1 epitopes in the CNS.

bioRxiv · May 11, 2026 · PreprintImmunology

Antigen-specificity of clonally-enriched CD8+ T cells in multiple sclerosis

Fumie Hayashi, Kristen Mittl, Ravi Dandekar, Josiah Gerdts, Ebtesam Hassan, Ryan D. Schubert, Lindsay Oshiro, Rita P. Loudermilk, Ariele L. Greenfield, Danillo G. Augusto, Gregory C Havton, Shriya Anumarlu, Arhan Surapaneni, Akshaya Ramesh, Edwina B. Tran, , Joseph J. Sabatino

Paired CSF and blood scRNA/TCR-seq in treatment-naive MS shows a small set of expanded, cytotoxic CSF-enriched CD8+ clonotypes, two of which recognize EBV epitopes.

Nature Immunology · Feb 5, 2026Peer-reviewedImmunology✦ In-depth digest

Antigen-Specific T Cell Receptor Discovery for Treating Progressive Multifocal Leukoencephalopathy

Sasha Gupta, Tijana Martinov, Ashley M. Thelen, Megumi Sunahara, Shwetha Mureli, Angie Vazquez, Josiah Gerdts, Ravi Dandekar, Irene C. M. Cortese, Camille Fouassier, Elaine V. Schanzer, Fyodor D. Urnov, Alexander Marson, Brian Rudolph Shy, Philip D. Greenberg, Michael R. Wilson

The paper identifies JCV VP1 epitopes recognized by HLA-A2 and isolates TCRs that recognize these epitopes, showing cytotoxic activity against infected cells.

bioRxiv · Nov 4, 2024 · PreprintNeuroscience

High-yield production of recombinant human myelin oligodendrocyte glycoprotein in SHuffle bacteria without a refolding step

Wesley C. H. Wu, Sasha Gupta, Sharon A. Sagan, Carson E. Moseley, Scott S. Zamvil, John E. Pak

This paper describes a method to produce soluble recombinant human MOG in SHuffle bacteria without refolding, enabling B cell-dependent EAE studies.

Journal of Immunological Methods · Oct 13, 2024Peer-reviewedBiochemistry