Angel Luis Pey

Minimized Sample Consumption for Time-Resolved Serial Crystallography Applied to the Redox Cycle of Human NQO1

This paper demonstrates a method to reduce sample consumption in time-resolved serial crystallography by using segmented droplet generation and mix-and-inject approaches for studying NQO1's redox cycle.

Phosphorylation of Ser81 in human AGT reversibly inactivate enzyme function and mimics catalytic defects of certain PH1-causing mutations

The paper shows phosphorylation of Ser81 in AGT inactivates the enzyme and mimics some PH1-causing mutations.

Nanobodies as therapies for loss-of-function misfolding diseases.

This study develops and characterizes six nanobodies as potential therapeutics for PH1 by targeting misfolded AGT variants, showing high affinity and functional enhancement in cellular models.

Engineered Oxalate decarboxylase boosts activity and stability for biological applications

This study engineers Oxalate decarboxylase to improve its activity and stability for medical applications in hyperoxaluria treatment.

Active site tyrosine residues in human NQO1 homodimer are critical for non-synchronous enzyme catalysis at the two active sites.

This study investigates the role of Tyr126 and Tyr128 in human NQO1 catalysis, showing their critical impact on enzyme activity and non-synchronous catalysis.

The lack of trade-off between conformational stability and binding affinity in a nanobody with therapeutic potential for a misfolding disease

This study shows that reducing the stability of a nanobody does not affect its binding affinity, suggesting stability and affinity can be decoupled in therapeutic proteins.

Thermodynamic versus kinetic basis for the high conformational stability of nanobodies for therapeutic applications

This study shows that nanobodies' thermal stability is primarily thermodynamic, using a two-state denaturation model across a wide temperature range.