Zu‐Wen Sun

The paper shows that different binding modes to the nucleosome acidic patch lead to distinct cellular outcomes, with overexpression of Dot5 and camelid antibodies causing growth, DNA repair, and heterochromatin defects.

This paper introduces Nuc-MS, a method to directly analyze chromatin reader-nucleosome interactions by disassembling complexes and identifying histone proteoforms involved in binding.

The paper provides a detailed CUT&RUN protocol for high-resolution epigenomic profiling, including best practices and controls for efficient target profiling.

The paper investigates how nucleosome context influences chromatin reader binding preferences using biochemical and genomic approaches, revealing that multivalent engagement often has regulatory effects beyond simple additivity.

The paper shows that histone reader domains interact with PTMs in a nucleosome-dependent manner, necessitating a refinement of the histone code concept to the nucleosome level.

The paper investigates the substrate preferences and functional consequences of Type I and II PRMTs by inhibiting them in A549 cells and analyzing proteomic and transcriptomic changes.