Martis W. Cowles

Different modes of engagement with the nucleosome acidic patch yield distinct functional outcomes

The paper shows that different binding modes to the nucleosome acidic patch lead to distinct cellular outcomes, with overexpression of Dot5 and camelid antibodies causing growth, DNA repair, and heterochromatin defects.

High-efficiency genomic mapping of chromatin-associated targets with CUT&RUN

The paper provides a detailed CUT&RUN protocol for high-resolution epigenomic profiling, including best practices and controls for efficient target profiling.

Nucleosome context regulates chromatin reader preference

The paper investigates how nucleosome context influences chromatin reader binding preferences using biochemical and genomic approaches, revealing that multivalent engagement often has regulatory effects beyond simple additivity.

Nucleosome conformation dictates the histone code

The paper shows that histone reader domains interact with PTMs in a nucleosome-dependent manner, necessitating a refinement of the histone code concept to the nucleosome level.