The paper models CRISPR homing gene drives with multiple gRNAs, finding optimal gRNA numbers and showing their limited standalone effectiveness but value when combined with other strategies.

135 citationstop 2%

statistical modelbenchmarking

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GeneticsNature CommunicationsPeer-reviewedFeb 27, 2020

A toxin-antidote CRISPR gene drive system for regional population modification

Jackson Champer, Esther Lee, Emily Yang…·Philipp W. Messer

The paper introduces a CRISPR gene drive system called TARE that limits resistance and spreads regionally in Drosophila.

134 citationstop 2%

Drosophilaexperimental assay

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GeneticsGeneticsPeer-reviewedMar 27, 2019

Multiple loci of small effect confer wide variability in efficiency and resistance rate of CRISPR gene drive

Jackson Champer, Zhaoxin Wen, Anisha Luthra…·Andrew G. Clark

The paper investigates how natural genetic variation affects resistance to CRISPR gene drives in Drosophila, finding that resistance rates vary widely and are polygenic with small-effect loci.

46 citations

DrosophilaCRISPR screen

𝕏 PostBluesky

GeneticseLifePeer-reviewedJan 21, 2019

Molecular safeguarding of CRISPR gene drive experiments

Jackson Champer, Joan Chung, Yoo Lim Lee…·Philipp W. Messer

The paper demonstrates synthetic target sites and split drives as safeguarding strategies to prevent unintended spread of CRISPR gene drives from lab to natural populations.

111 citationstop 3%

experimental assay

𝕏 PostBluesky

GeneticsPLoS GeneticsPeer-reviewedJul 20, 2017

Novel CRISPR/Cas9 gene drive constructs in Drosophila reveal insights into mechanisms of resistance allele formation and drive efficiency in genetically diverse populations

Jackson Champer, Riona Reeves, Suh Yeon Oh…·Philipp W. Messer

295 citationstop 1%

Drosophilaexperimental assay

𝕏 PostBluesky