Emily Yang

New germline Cas9 promoters show improved performance for homing gene drive

The paper identifies new germline Cas9 promoters in Drosophila that improve drive conversion efficiency while reducing embryo resistance and somatic expression.

A homing suppression gene drive with multiplexed gRNAs maintains high drive conversion efficiency and avoids functional resistance alleles

This paper develops a homing suppression gene drive in Drosophila using multiplexed gRNAs to prevent resistance alleles, achieving high conversion efficiency and moderate population suppression.

Experimental demonstration of tethered gene drive systems for confined population modification or suppression

This paper demonstrates a tethered gene drive system in Drosophila that confines homing drives to target populations, enabling controlled modification or suppression.

Resistance is futile: A CRISPR homing gene drive targeting a haplolethal gene

This paper presents a CRISPR homing gene drive in Drosophila that reduces resistance allele formation by targeting a haplolethal gene, achieving high efficiency in allele spreading.

A toxin-antidote CRISPR gene drive system for regional population modification

The paper introduces a CRISPR gene drive system called TARE that limits resistance and spreads regionally in Drosophila.

Multiple loci of small effect confer wide variability in efficiency and resistance rate of CRISPR gene drive

The paper investigates how natural genetic variation affects resistance to CRISPR gene drives in Drosophila, finding that resistance rates vary widely and are polygenic with small-effect loci.

Molecular safeguarding of CRISPR gene drive experiments

The paper demonstrates synthetic target sites and split drives as safeguarding strategies to prevent unintended spread of CRISPR gene drives from lab to natural populations.