Author

Sanne E. Klompe

Columbia UniversityORCID 0000-0002-0151-9459Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

Click to filter author papers

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:experimental assay· 3 Assay:CRISPR screen· 2 Organism:bacteria (other)· 1 Research Focus:gene regulatory network inference· 1 Research Focus:genome assembly· 1 Organism:human· 1 Research Focus:software / tool· 1

Frequent Co-Authors

Samuel H. SternbergiD· 3 shared Phuc Leo H. VoiD· 2 shared Alejandro ChaveziD· 1 shared Carlotta RondaiD· 1 shared Christopher AcreeiD· 1 shared Dennis J. ZhangiD· 1 shared Ethan E. CheniD· 1 shared George D. LampeiD· 1 shared

Activity by Year

2020-2023 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

3 shared papersGenetics

Samuel H. SternbergORCID

Mutual co-authors in cluster

Author profile →

3 papers

Recent·Most cited

Transposon mutagenesis libraries reveal novel molecular requirements during CRISPR RNA-guided DNA integration

Matt W.G. Walker, Sanne E. Klompe, Dennis J. Zhang, Samuel H. Sternberg

This study identifies sequence requirements for CRISPR-associated transposon DNA integration using mutagenesis libraries and reveals a novel role for IHF in transposition.

Nucleic Acids Research · Apr 20, 2023Peer-reviewedGenetics

Targeted DNA integration in human cells without double-strand breaks using CRISPR RNA-guided transposases

George D. Lampe, Rebeca T. King, Tyler S. Halpin-Healy, Sanne E. Klompe, Marcus I. Hogan, Phuc Leo H. Vo, Stephen Tang, Alejandro Chavez, Samuel H. Sternberg

This paper develops a CRISPR-guided transposon system for DSB-free DNA integration in human cells, improving efficiency and reducing off-target effects.

Nature Biotechnology · Mar 29, 2023Peer-reviewedGenetics

CRISPR RNA-guided integrases for high-efficiency and multiplexed bacterial genome engineering

Phuc Leo H. Vo, Carlotta Ronda, Sanne E. Klompe, Ethan E. Chen, Christopher Acree, Harris H. Wang, Samuel H. Sternberg

This paper introduces an improved CRISPR-based system for high-efficiency, multiplexed bacterial genome engineering using RNA-guided integrases.

Nature Biotechnology · Nov 23, 2020Peer-reviewedMolecular Biology