Samuel H. Sternberg

The paper identifies Arc as a transcriptional repressor in IS605-family transposons, showing it binds promoters to regulate TnpA and TnpB expression.

The paper identifies a TldR repressor in Enterococcus faecalis that directly represses OppA, revealing its role in regulating bacterial peptide import and uncovering a conserved structural mechanism inherited from TnpB ancestors.

This study investigates the mechanism of transposon excision by type I-F CRISPR-associated transposases using structural and biochemical analyses.

The paper identifies molecular mechanisms governing dual RNA functions in transposons through mutational analysis and high-throughput sequencing.

The paper shows how two reverse transcriptases with different templating strategies work together to produce complementary DNA for antiviral defense.

The paper investigates the mechanism of tandem-repeat DNA synthesis by DRT10, revealing a 2:1 RT-ncRNA architecture and how repeat length is controlled by template distance.

This study shows that temperate phages enhance bacterial fitness by remodeling flagella via RNA-guided transcription factors, improving motility and immune evasion.

This study uses cryo-EM to reveal the structural mechanism of RNA-guided DNA integration by PseCAST, providing insights for engineering DNA insertion systems.

The paper identifies a novel RNA-guided transcription mechanism using dCas12f and σE factors in E. coli, enabling gene activation without traditional promoter motifs.

This study determines cryo-EM structures of the dCas12f-σE-RNAP complex to reveal a novel RNA-guided transcription mechanism in bacteria.

This study reveals the structural basis and evolutionary origin of the Lamassu bacterial immune system, showing its adaptation from DNA repair machinery to detect and respond to viral replication.

The paper identifies DRT10, an antiviral reverse transcriptase, and shows its structural and mechanistic similarities to telomerase, suggesting a bacterial origin for telomerase.

This paper engineers type I-F CAST systems for targeted gene insertion in human cells by combining structural insights with rational design to improve efficiency and PAM flexibility.

This study investigates the DNA recognition properties of transposases in Oxytricha trifallax using heterologous assays and ChIP-seq, revealing relaxed sequence specificity and precise nuclear localization during genome rearrangement.

The paper reveals a novel DNA homopolymer synthesis mechanism by DRT9, triggered by viral infection to induce abortive infection and population-level immunity.

This study identifies evolutionary links between bacterial IS110 transposons and archaeal/eukaryotic RNA modification systems, suggesting RNA-guided mechanisms evolved from transposon components.

The paper demonstrates that bacterial reverse transcriptases synthesize extrachromosomal genes via rolling-circle amplification to confer antiviral immunity.

The paper investigates the molecular mechanisms of transposition and RNA-guided DNA cleavage in IS607-family elements, revealing how transposon-encoded RNAs balance splicing and guide RNA functions.

The paper identifies TnpB-like nuclease-dead repressors (TldR) as RNA-guided transcription factors that emerged through domestication of transposon-encoded TnpB nucleases, pre-dating CRISPR-Cas systems.

This study identifies two mobilization pathways in type V-K CASTs and shows how manipulating TnsC availability can increase their specificity to 98.1%.

This study shows that transposon-encoded nucleases use guide RNAs to bias transposon inheritance by cleaving donor joints formed during transposition.

This study identifies sequence requirements for CRISPR-associated transposon DNA integration using mutagenesis libraries and reveals a novel role for IHF in transposition.

This paper develops a CRISPR-guided transposon system for DSB-free DNA integration in human cells, improving efficiency and reducing off-target effects.

The paper uses long-read sequencing to analyze transposition products of RNA-guided transposons, finding they primarily use copy-and-paste mechanisms and report engineered variants with TnsAB fusion proteins.

This paper introduces an improved CRISPR-based system for high-efficiency, multiplexed bacterial genome engineering using RNA-guided integrases.