George D. Lampe

Transposon end recognition and excision mechanisms of type I-F CRISPR-associated transposases

This study investigates the mechanism of transposon excision by type I-F CRISPR-associated transposases using structural and biochemical analyses.

Mechanism of tandem-repeat DNA synthesis by an antiviral reverse transcriptase

The paper investigates the mechanism of tandem-repeat DNA synthesis by DRT10, revealing a 2:1 RT-ncRNA architecture and how repeat length is controlled by template distance.

Structural basis of RNA-guided DNA integration by type I CRISPR-associated transposases

This study uses cryo-EM to reveal the structural mechanism of RNA-guided DNA integration by PseCAST, providing insights for engineering DNA insertion systems.

Exapted CRISPR-Cas12f homologs drive RNA-guided transcription

The paper identifies a novel RNA-guided transcription mechanism using dCas12f and σE factors in E. coli, enabling gene activation without traditional promoter motifs.

TGF-β induces an atypical EMT to evade immune mechanosurveillance in lung adenocarcinoma dormant metastasis

This study identifies an atypical EMT induced by TGF-β in lung adenocarcinoma metastatic progenitors that reduces biomechanical stiffness, enabling evasion of immune surveillance during dormancy.

Structure-guided engineering of type I-F CASTs for targeted gene insertion in human cells

This paper engineers type I-F CAST systems for targeted gene insertion in human cells by combining structural insights with rational design to improve efficiency and PAM flexibility.

Protein-primed DNA homopolymer synthesis by an antiviral reverse transcriptase

The paper reveals a novel DNA homopolymer synthesis mechanism by DRT9, triggered by viral infection to induce abortive infection and population-level immunity.

De novo gene synthesis by an antiviral reverse transcriptase

The paper demonstrates that bacterial reverse transcriptases synthesize extrachromosomal genes via rolling-circle amplification to confer antiviral immunity.

Antagonistic conflict between transposon-encoded introns and guide RNAs

The paper investigates the molecular mechanisms of transposition and RNA-guided DNA cleavage in IS607-family elements, revealing how transposon-encoded RNAs balance splicing and guide RNA functions.

Targeted DNA integration in human cells without double-strand breaks using CRISPR RNA-guided transposases

This paper develops a CRISPR-guided transposon system for DSB-free DNA integration in human cells, improving efficiency and reducing off-target effects.