Rafael Donadelli Melani

This paper improves ETD-based fragmentation for glycopeptide analysis by optimizing ion handling and scan parameters, making it faster and more efficient for both N- and O-glycopeptides.

This paper introduces a high-resolving power DIA-PTCR method for glycoproteoform analysis, enabling isotopically resolved mass measurements and integration with other glycoproteomics approaches.

This study benchmarks sub-50 nm quartz nanopipette emitters for native electrospray ionization mass spectrometry across multiple platforms and protein sizes, showing improved signal-to-noise and reduced adduction.

This study revisits resonance-excitation collision-induced dissociation (reCID) for DIA proteomics, showing it can achieve performance comparable to HCD with optimized settings and libraries.

The paper introduces REVEAL, a mass spectrometry method to identify receptor-specific lipid binding in mammalian membrane proteins, showing agonism alters lipid occupancy and plasmalogen lipids are depleted under oxidative stress.

This study introduces ProteoMIX, a native top-down proteomics method to identify hemophores in C. diphtheriae, revealing protease-mediated release of soluble heme-binding proteoforms.

This study uses top-down proteomics to identify plasma proteoform signatures that distinguish stages of liver cirrhosis progression.

This study integrates transcriptomic, proteomic, and genomic data from CZS neonates to identify collagen gene reductions and associated polymorphisms, linking them to neuronal migration defects and CZS susceptibility.