Zhaohan Zhu

Exposure Duration Shapes the Hepatic Response to GenX: Divergent Acute and Chronic Transcriptomic Profiles Reveal Non-Monotonic Dose Effects and Increased Sensitivity in Human Liver Spheroids

simCRISPR: Modeling experimental complexity in pooled CRISPR screens

The paper develops simCRISPR, a simulation framework for pooled CRISPR screens, evaluates normalization strategies for detecting gene-by-environment interactions, and shows improved results with safe-harbor normalization.

Physiologically Relevant 3D CRISPR Screening Enhances Mechanistic Insight into Chemical Toxicity Compared to 2D Screening

This paper develops a 3D CRISPR screening system using HepG2/C3A spheroids to improve the identification of genetic determinants of chemical toxicity compared to traditional 2D methods.

Identification of Functional Genetic Components Modulating Toxicity Response to PFOS using Genome-wide CRISPR Screens in HepG2/C3A cells

This study identifies 340 genes modulating PFOS-induced cytotoxicity in liver cells using CRISPR screens and validates two genes, SLC6A9 and CPSF2, with functional and molecular evidence.

Genome-wide CRISPR screens identified C18orf32 as a novel regulator of lipid metabolism that mediates PFOA-induced toxicity

The paper identifies C18orf32 as a novel regulator of lipid metabolism involved in PFOA-induced toxicity through CRISPR screening and transcriptomic analysis.

Characterizing common loss-of-function genes and their potential utility in assessing population variability and chemical susceptibility

The paper identifies common loss-of-function genes and develops a method to integrate them into cell-based models to assess population variability in chemical susceptibility.