Gaurav K. Varshney

This study identifies a naturally evolved mutation in mc3r that alters energy homeostasis across development in cavefish, contributing to adaptation to nutrient scarcity.

The paper generates a systematic in vivo atlas of loss-of-function alleles in zebrafish mitochondrial genes, revealing their impact on bioenergetics and hair cell function.

The paper identifies bi-allelic ATG12 variants causing a neurodevelopmental disorder by impairing autophagy, with clinical and functional evidence from patient cells, yeast, and zebrafish models.

This study identifies XPOT deficiency as a human disorder causing immune defects, hearing loss, and developmental issues through impaired tRNA nuclear export.

This study identifies biallelic TRMT1 variants causing a syndromic neurodevelopmental disorder through tRNA modification defects in humans and zebrafish.

The paper introduces zTadCBE, a TadA-derived cytosine base editor for precise genome editing in zebrafish, showing high efficiency, reduced off-target effects, and expanded targeting range.

This study shows that zebrafish Naa80 acetylates actins and is essential for normal hearing, but not for muscle development or viability.

This study shows that cnr2 is critical for ribbon synapse maturation and function in hair cells and photoreceptors, affecting synaptic structure and behavior in zebrafish larvae.

This study identifies biallelic mutations in CLRN2 as a cause of non-syndromic hearing loss through genetic analysis of a consanguineous family and functional validation in zebrafish and mouse models.

The paper generates zebrafish CRISPR mutants for DNA repair genes and identifies their roles in development and disease.

This study shows that appb mutation in zebrafish causes early developmental defects but allows normal adult development, suggesting compensation by other app family members.

The paper develops MultiFRAGing, a method for rapid and simultaneous genotyping of multiple alleles using fluorescent PCR-based fragment analysis in zebrafish CRISPR experiments.

The paper describes a zebrafish gene break transposon library for genomic discovery and disease modeling, identifying novel gene expression patterns and generating models for human disease loci.

This study identifies GATA2's role in maintaining lymphatic endothelial cell junctions and valve morphogenesis through regulation of miR-126.

This study shows that Cpf1 can achieve high gene targeting rates in mice, sometimes surpassing Cas9, but requires higher mRNA and guide concentrations.