Ulschan Bathe

This paper demonstrates how mass spectrometry-based proteomics can be used in continuous directed evolution to analyze protein modifications and network integration during evolution.

The paper reports the production of 130 diterpenoids in yeast through combinatorial biosynthesis using ten diterpene synthases and four cytochrome P450 enzymes, with 80 novel compounds identified.

The paper describes a method to improve enzymes for plant applications using continuous directed evolution in yeast, demonstrating its effectiveness with a bacterial thiamin synthesis enzyme.