Keith P. Romano

The paper develops a CRISPRi system for studying essential genes in Pseudomonas aeruginosa, revealing gene function and small molecule interactions through phenotypic analysis.

The paper identifies a Pseudomonas aeruginosa-specific small molecule, BRD1401, that targets OprH and disrupts its interaction with LPS, increasing membrane fluidity.

This paper introduces PerSpecTM, a method to predict antibiotic mechanisms of action by comparing transcriptional responses of new molecules to known perturbations, demonstrating its effectiveness with three new anti-Pseudomonas aeruginosa compounds.

This study investigates how the distal mutation V36M confers drug resistance in HCV NS3/4A protease through allosteric modulation of the active site, in the context of R155K.