Nigel J. Savery

In-vivo distributed multicellular control of gene expression in microbial consortia

This paper develops a distributed feedback control system in microbial consortia to precisely regulate gene expression across different cell populations.

From peptides to proteins: coiled-coil tetramers to single-chain 4-helix bundles

The paper designs synthetic proteins by deriving sequence-to-structure rules from coiled-coil tetramers and validates them through experimental methods.

Cheetah: a computational toolkit for cybergenetic control

Cheetah is a computational toolkit that integrates real-time microscopy analysis with cellular control algorithms, demonstrating improved segmentation accuracy over thresholding methods in bacterial and mammalian cells.

ChipSeg: an automatic tool to segment bacteria and mammalian cells cultured in microfluidic devices

ChipSeg segments bacterial and mammalian cells in microfluidic devices using thresholding, enabling fluorescence quantification and cell tracking in time-lapse experiments.

In vivo Feedback Control of an Antithetic Molecular-Titration Motif in Escherichia coli using Microfluidics

The paper demonstrates real-time feedback control of a molecular titration motif in E. coli using microfluidics and validates the approach with in silico simulations.

Guiding biomolecular interactions in cells using de novo protein-protein interfaces

This paper designs de novo protein-protein interfaces to control biomolecular interactions in E. coli, demonstrating their ability to guide engineered protein-DNA interactions and modulate gene expression.

A low cost, open source Turbidostat design for in-vivo control experiments in Synthetic Biology

The paper describes a low-cost, open-source turbidostat for in-vivo control experiments in synthetic biology and reports results from a 3-hour optical density control experiment.