This paper introduces a method using macrocyclic phage display to identify selective protease substrates, demonstrating its effectiveness with FAP and DPP4.

Journal of the American Chemical Society · Jul 18, 2025Peer-reviewedBiochemistry

Identification of Covalent Cyclic Peptide Inhibitors Targeting Protein-Protein Interactions Using Phage Display

Sijie Wang, Franco F. Faucher, Matilde Bertolini, Heeyoung Kim, Bingchen Yu, Li Cao, Katharina Röltgen, Scott Lovell, Varun R. Shanker, Scott Dexter Boyd, Lei Wang, Ralf F. W. Bartenschlager, Matthew S. Bogyo

This paper identifies covalent cyclic peptide inhibitors targeting protein-protein interactions using phage display, demonstrating antiviral activity against SARS-CoV-2.

Journal of the American Chemical Society · Feb 24, 2025Peer-reviewedMicrobiology

Polymer-tethered quenched fluorescent probes for enhanced imaging of tumor associated proteases

Martin Hadzima, Franco F. Faucher, Kristýna Blažková, Joshua J. Yim, Matteo Guerra, Shiyu Chen, Emily C. Woods, Ki Wan Park, Pavel Šácha, Vladimír Šubr, Libor Kostka, Tomáš Etrych, Pavel Majer, Jan Konvalinka, Matthew S. Bogyo

This paper develops polymer-tethered fluorescent probes for tumor-associated protease imaging, showing improved signal and tumor-to-background ratios compared to small molecule probes.

ACS Sensors · Jun 28, 2024Peer-reviewedBioengineering