Eleftherios Terry Papoutsakis

The paper develops a two-step bioprocess using Clostridium species to convert C1-C2 CO2RR products into C4-C6 chemicals, improving carbon efficiency.

This study shows that late-stage large extracellular vesicles (LgEVs) from CHO cells reduce growth but increase antibody productivity, with glutamine modulating these effects through metabolic and miRNA mechanisms.

This paper validates a pseudo perfusion platform for CHO cells to generate reliable metabolic data and guide continuous perfusion biomanufacturing, showing it predicts amino acid demands and maintains steady states.

This study shows that high cell density cocultures of genetically modified Clostridium acetobutylicum and wild-type Clostridium ljungdahlii enable supratheoretical isopropanol yields from glucose fermentation.

The paper develops a DMFA model to predict CHO cell culture performance under various conditions and validates its predictions experimentally.

This study shows that H2 solubility limits C. ljungdahlii growth on CO2 and H2, but high H2 mass transfer enables rapid growth using CO2 as the sole carbon source.

This paper shows that engineering nitrogen exchange in a mixotrophic coculture of Clostridium acetobutylicum and Clostridium ljungdahlii enables mutualism, stabilizes population ratios, and improves product yields.

This study enhances isopropanol and butanol production by engineering microbial syntrophic cocultures of Clostridium acetobutylicum and Clostridium ljungdahlii, achieving high yields and efficiency.

The paper uses RNA sequencing to compare gene expression in syntrophic cocultures of Clostridium acetobutylicum and Clostridium ljungdahlii to monocultures and separated cocultures, identifying genes involved in metabolism and cell fusion.

This paper develops and applies species-specific rRNA-FISH to study interspecies material exchange and population dynamics in clostridial co-cultures, revealing hybrid cell formation and distinct translation machinery localization.

This study examines how bioreactor pH affects CHO cell metabolism and N-linked glycosylation of IgG antibodies using flux balance analysis and glycopeptide mapping.

This study shows that butyrate stimulates growth, health, and sporulation in Clostridium acetobutylicum and acts as a growth factor, potentially modulating microbial population dynamics.