This study identifies pfaat1 as a key gene in chloroquine resistance evolution in malaria parasites, showing it modulates resistance and fitness through amino acid transport.

bioRxiv · May 26, 2022 · PreprintGenomics

Optimizing bulk segregant analysis of drug resistance using Plasmodium falciparum genetic crosses conducted in humanized mice

Katelyn M. Vendrely, Xue Li, Sudhir Kumar, Elizabeth Delgado, Lisa A. Checkley, Douglas A. Shoue, Ann N. Reyes, Biley A. Abatiyow, Meseret T. Haile, Rupam Tripura, Thomas Julian Peto, Dysoley Lek, Katrina A. Button-Simons, Stefan H. I. Kappe, Mehul Dhorda, , Tim Anderson

This study optimizes bulk segregant analysis for identifying drug resistance loci in Plasmodium falciparum using genetic crosses in humanized mice, confirming the kelch13 locus and demonstrating the utility of cryopreserved pools.

iScience · Mar 16, 2022Peer-reviewedGenomics

Bulk segregant analysis reveals environment x genotype interactions determining malaria parasite growth

Sudhir Kumar, Xue Li, Marina McDew‐White, Ann N. Reyes, Elizabeth Delgado, Abeer Sayeed, Meseret T. Haile, Biley A. Abatiyow, Spencer Y. Kennedy, Nelly M. Camargo, Lisa A. Checkley, Katelyn V. Brenneman, Katrina A. Button-Simons, Manoj T. Duraisingh, Ian H. Cheeseman, , Tim Anderson

The paper uses bulk segregant analysis to identify genetic loci affecting malaria parasite growth in different media, finding EBA-140 as a key gene involved in media-dependent fitness.

bioRxiv · Sep 12, 2020 · PreprintGenetics