Author

Tomáš Knotek

Czech Academy of SciencesORCID 0000-0002-1296-155XUnclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Research Focus:cell type annotation· 3 Organism:mouse· 3 Assay:bulk RNA-seq· 2 Approach:statistical model· 2 Research Focus:benchmarking· 1 Approach:experimental assay· 1 Assay:scRNA-seq· 1

Frequent Co-Authors

Denisa KirdajováiD· 3 shared Ján KriškaiD· 3 shared Miroslava AnděrováiD· 3 shared Jan KubovčiakiD· 2 shared Jana TurečkováiD· 2 shared Lucie JanečkováiD· 2 shared Michal KolářiD· 2 shared Vladimír KořínekiD· 2 shared

Activity by Year

2023-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 39 mutual co-author links

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3 shared papersNeuroscience

Denisa KirdajováORCID

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3 papers

Recent·Most cited

NeuroscienceCommunications BiologyPeer-reviewedAug 26, 2026

Canonical Wnt signaling controls the fate and plasticity of NG2 glia in the healthy and ischemic adult mouse cortex

Tomáš Knotek, Lucie Janečková, Ján Kriška…·Vladimír Kořínek

This study shows Wnt signaling regulates NG2 glia fate in adult mouse cortex, promoting neuronal conversion while impairing oligodendrocyte maturation after ischemic injury.

DOI 10.1038/s42003-026-10813-9

mousebulk RNA-seq

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NeurosciencebioRxivPreprintAug 11, 2025

PU.1-driven enrichment enables microglia profiling from frozen brain tissue using the high-throughput Smart-seq3xpress method

Dominika Dostalova, Pavel Abaffy, Eva Rohlová…·Lukáš Valihrach

The paper introduces a method to profile microglia from frozen brain tissue using PU.1 as a marker and Smart-seq3xpress, showing improved transcriptomic data compared to standard protocols.

DOI 10.1101/2025.08.11.669607

mousescRNA-seq

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NeuroscienceGliaPeer-reviewedSep 29, 2023

Astrocyte-like subpopulation of NG2 glia in the adult mouse cortex exhibits characteristics of neural progenitor cells and is capable of forming neuron-like cells after ischemic injury

Lucie Janečková, Tomáš Knotek, Ján Kriška…·Vladimír Kořínek

The paper identifies an astrocyte-like NG2 glia subpopulation in adult mouse cortex that shows neurogenic potential and can form neuron-like cells after ischemic injury.

25 citations

mousebulk RNA-seq

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