Assessing cellular metabolic dynamics with NAD(P)H fluorescence polarization imaging
Ling Lu, Jack C. Crowley, Matthew L. Tan, Jennie A. M. R. Kunitake, Adrian A. Shimpi, Rebecca M. Williams, Lara A. Estroff, Claudia Fischbach, Warren R. Zipfel
This paper introduces FPRM, a fast, label-free imaging method to monitor cellular metabolism using NAD(P)H autofluorescence, and demonstrates its application in tracking metabolic changes under various perturbations.
bioRxiv · Jul 28, 2025 · PreprintBiophysics