Megha Chandrashekhar

A global genetic interaction map of a human cell reveals conserved principles of genetic networks

The paper constructs a genome-scale genetic interaction network from 4 million double mutants in HAP1 cells, revealing conserved genetic network principles across species and uncovering novel gene functions and cancer dependencies.

Evaluation and Design of Genome-wide CRISPR/Cas9 Knockout Screens

The paper evaluates CRISPR/Cas9 knockout screens in human cell lines and designs an optimized gRNA library to improve gene knockout efficiency and accuracy.

A CRISPR screen reveals a WNT7B-FZD5 signaling circuit as a therapeutic opportunity in pancreatic cancer.

The paper identifies a WNT7B-FZD5 signaling circuit as a therapeutic target in pancreatic cancer using CRISPR screening and validates it with antibodies.

Systematic discovery and classification of human cell line essential genes

This study identifies and classifies human cell line essential genes using an optimized CRISPR-Cas9 screen, finding twice to thrice as many essential genes as previous RNAi studies.