Zachary Steinhart

Virus-like particles enable targeted gene engineering and pooled CRISPR screening in primary human myeloid cells

This paper develops a virus-like particle-based system for CRISPR gene editing and screening in primary human myeloid cells, identifying TNFAIP3 as a key regulator of inflammatory polarization.

A genome-wide CRISPR activation map of surface protein expression in human CD4 T cells

This paper introduces SCITO-Perturb-seq, a CRISPRa platform that maps surface protein regulation in CD4 T cells, finding 16% of activated genes alter surface protein expression.

Systematic profiling of the acetyl lysine machinery reveals a role for MAPKAPK2 in bromodomain inhibitor resistance

This study maps the acetyl lysine machinery interactions and identifies MAPKAPK2 as a factor contributing to resistance to BET bromodomain inhibitors in melanoma.

Bidirectional epigenetic editing reveals hierarchies in gene regulation

This study introduces CRISPRai, a bidirectional epigenetic editing system, to investigate gene regulation hierarchies and interactions in T cells and hematopoietic lineage transcription factors.

Single molecule dynamics of Dishevelled at the plasma membrane and Wnt pathway activation

This study uses single-molecule imaging to analyze Dvl2 dynamics and oligomerization during Wnt pathway activation, showing increased oligomerization and dwell time upon Wnt3a stimulation.

The functional genomic circuitry of human glioblastoma stem cells

This study uses CRISPR-Cas9 screens in patient-derived glioblastoma stem cells to identify essential genes and pathways for stemness and drug resistance, revealing new therapeutic targets.

A CRISPR screen reveals a WNT7B-FZD5 signaling circuit as a therapeutic opportunity in pancreatic cancer.

The paper identifies a WNT7B-FZD5 signaling circuit as a therapeutic target in pancreatic cancer using CRISPR screening and validates it with antibodies.

Systematic discovery and classification of human cell line essential genes

This study identifies and classifies human cell line essential genes using an optimized CRISPR-Cas9 screen, finding twice to thrice as many essential genes as previous RNAi studies.