Thomas Klose

Agentic cryo-EM structure determination with CryoPilot

CryoPilot uses two language model agents to automate cryo-EM structure determination, improving resolution and model accuracy through adaptive decision-making.

Analysis of the heterogenous structural states of the hexameric ATPase PilU of the Type IV pili from Vibrio cholerae

The paper determines six structural states of PilU, a retraction ATPase, revealing its homohexameric assembly and conformational flexibility, which may explain its weak ATPase activity and dependence on PilT.

An unconventional HxD motif orchestrates coatomer-dependent coronavirus morphogenesis

The paper identifies an unconventional HxD motif in coronaviruses that functions as a coatomer-binding signal, revealing alternative pathways for viral morphogenesis.

Structure of Vibrio phage XM1, a simple contractile DNA injection machine

The paper reports the cryo-EM structures of Vibrio phage XM1, revealing its contractile tail and baseplate components, and proposes mechanisms of assembly and infection.