Gaia Pigino

In situ structures of the ciliary tip show the multi-step mechanism of axoneme assembly

The paper visualizes axoneme assembly in situ using cryo-electron tomography and identifies conserved microtubule luminal proteins and assembly intermediates.

Unwrapping the ciliary coat: high-resolution structure and function of the ciliary glycocalyx

This study determines the high-resolution structure of the ciliary glycocalyx in Chlamydomonas reinhardtii and identifies FMG1B as a mucin-like protein with unique glycosylation features.

MicroSplit: Semantic Unmixing of Fluorescent Microscopy Data

MicroSplit uses deep learning to computationally unmix fluorescent microscopy data, enabling faster imaging and reduced photon exposure by separating multiple cellular structures from a single channel.

Tubulin tyrosination/detyrosination governs the spatial segregation of intraflagellar transport trains on axonemal microtubule doublets

This study shows that tubulin tyrosination/detyrosination governs the spatial segregation of IFT trains on ciliary microtubules, preventing traffic jams.

The Molecular Structure of Anterograde Intraflagellar Transport Trains

This study uses cryo-electron tomography and AlphaFold2 to model the structure of anterograde intraflagellar transport trains and elucidate their polymerization mechanism.

The molecular structure of primary cilia revealed by cryo-electron tomography

This study reveals the molecular structure of primary cilia using cryo-electron tomography, showing a transition from a 9+0 microtubule arrangement to an unstructured bundle of microtubule singlets and actin filaments.