The paper investigates how liver fluke granulin promotes exosome-mediated crosstalk that alters the cellular microenvironment to support cholangiocarcinoma development.

Neoplasia · Mar 31, 2020Peer-reviewedMolecular Biology

Programmed genome editing of the omega-1 ribonuclease of the blood fluke, Schistosoma mansoni

Wannaporn Ittiprasert, Victoria H. Mann, Shannon E. Karinshak, Avril Coghlan, Gabriel Rinaldi, Geetha Sankaranarayanan, Apisit Chaidee, Toshihiko Tanno, Chutima Kumkhaek, Pannathee Prangtaworn, Margaret M. Mentink‐Kane, Christina J. Cochran, Patrick Driguez, Nancy E. Holroyd, Alan Tracey, , Paul J. Brindley

This paper demonstrates CRISPR/Cas9-based genome editing in Schistosoma mansoni, successfully mutating the omega-1 gene and reducing Th2 cytokine responses and granuloma formation.

eLife · Jan 14, 2019Peer-reviewedMolecular Biology

Programmed mutation of liver fluke granulin using CRISPR/Cas9 attenuates virulence of infection-induced hepatobiliary morbidity

Patpicha Arunsan, Wannaporn Ittiprasert, Michael J. Smout, Christina J. Cochran, Victoria H. Mann, Sujittra Chaiyadet, Shannon E. Karinshak, Banchob Sripa, Neil David Young, Javier Sotillo, Alex C. Loukas, Paul J. Brindley, Thewarach Laha

This study uses CRISPR/Cas9 to knockout the Ov-grn-1 gene in liver flukes, showing reduced biliary tract pathology in infected hamsters.

eLife · Jan 14, 2019Peer-reviewedMolecular Biology