Philipp Holliger

This paper demonstrates a system using DGRs in E. coli to perform continuous, targeted mutagenesis of genes, enabling rapid evolution of specific sequences.

This study investigates untemplated DNA synthesis by polymerases, showing that environmental conditions influence sequence composition and providing data for guided DNA synthesis.

The paper describes a ribozyme that can synthesize both itself and its complementary strand, suggesting polymerase ribozymes may be more common in RNA sequence space.

This paper introduces gRNAde, an RNA language model for inverse design of RNA structure and function, achieving success rates comparable to human experts in designing pseudoknotted RNAs.

This study uses cryo-EM to characterize dimeric intermediates in RNA-catalyzed rolling circle synthesis, revealing their structural diversity and potential role in RNA replication.

RNA trinucleotide substrates and physicochemical cycles enable RNA-catalysed replication of double-stranded RNA and partial self-replication of an RNA polymerase ribozyme.

This study shows that functional RNAs can emerge from a single RNA sequence through mutation and selection, without requiring large random sequence pools.