Evan Pepper-Tunick

EMS Mutation and SNP Detection in Intracellular Wolbachia Genomes

This paper demonstrates the successful detection of EMS-induced mutations in Wolbachia genomes using circle sequencing, revealing a significant enrichment of C/G>T/A transitions.

Ultra-accurate sequencing reveals an extreme transmission bottleneck in a deep-sea clam symbiosis

The paper estimates an extreme transmission bottleneck in a deep-sea clam symbiosis using ultra-accurate sequencing, finding an effective bottleneck size of about eight symbionts per host generation.

Host oxidative stress primes mycobacteria for rapid antibiotic resistance evolution

This study shows that host oxidative stress primes Mycobacterium tuberculosis for rapid antibiotic resistance evolution by creating permissive genomic backgrounds that accelerate resistance mutations.

Mixed Wolbachia infections resolve rapidly during in vitro evolution

This study shows that the wMel Wolbachia strain outcompetes the promiscuous wRi strain in vitro across different host cell types, suggesting a deterministic outcome that may explain low natural mixed infections.

Chromosome-scale genome assembly of a single outbred Drosophila melanogaster

The paper demonstrates a chromosome-scale genome assembly of a single outbred Drosophila melanogaster using a hybrid method with low input DNA.