Makoto Ikeya

Capturing self-renewing multipotent neural crest stem cells from human pluripotent stem cells

This study establishes a culture system using Wnt and FGF signaling to expand human iPSC-derived neural crest stem cells long-term, maintaining their self-renewal and differentiation potential.

Induction and long-term maintenance of hindbrain-like neural stem cells in xeno- and basic fibroblast growth factor-free conditions

This study generates and maintains hindbrain-like neural stem cells without xeno- or basic fibroblast growth factors using three chemicals, showing long-term stability and potential for disease modeling and therapy.

Species-specific oscillation periods of human and mouse segmentation clocks are due to cell autonomous differences in biochemical reaction parameters

Mouse and human segmentation clocks oscillate at 2-3 h and 5-6 h; slower HES7 degradation and longer delays in human cells, not sequence, explain it.

Modeling the Human Segmentation Clock with Pluripotent Stem Cells

This study models the human segmentation clock using iPSC-derived presomitic mesoderm, identifying oscillatory genes and validating disease-related gene effects.