Simon Butterworth

The paper uses crosslinking mass spectrometry to map protein interactions in Toxoplasma gondii, identifying novel components of essential complexes and revealing structural diversity in mitochondrial machinery.

The paper identifies GRA12 as a critical secreted virulence factor in Toxoplasma gondii that functions across different parasite strains and mouse species.

The paper shows that MYR1-deficient Toxoplasma gondii mutants are rescued by co-infection with wild-type parasites in mice, revealing a limitation of pooled in vivo CRISPR screens.

This paper introduces dual perturb-seq, a method combining CRISPR screening and single-cell RNA-sequencing to identify host-microbe transcriptional interactions, revealing new effector proteins in Toxoplasma gondii.