Sebastian Lourido

Proteome-wide crosslinking mass spectrometry reveals novel components of essential complexes in Toxoplasma

The paper uses crosslinking mass spectrometry to map protein interactions in Toxoplasma gondii, identifying novel components of essential complexes and revealing structural diversity in mitochondrial machinery.

Brieflow: An Integrated Computational Pipeline for High-Throughput Analysis of Optical Pooled Screening Data

Brieflow is a computational pipeline for analyzing optical pooled screening data, demonstrating its ability to recover biological modules missed by existing methods.

Two distinct SWI/SNF complexes direct chromatin-linked transcriptional programs in Toxoplasma

The paper identifies two distinct SWI/SNF complexes in Toxoplasma gondii that have different roles in transcriptional regulation during the cell cycle and development.

Brain-immune interactions generate pathogen-specific sickness states

The paper investigates pathogen-specific sickness states by analyzing immune and neural responses across multiple scales in various inflammatory models.

Clustering of host N-glycans licenses Toxoplasma rhoptry discharge

The paper identifies host N-glycosylation and cholesterol biosynthesis as required for Toxoplasma rhoptry discharge, mediated by the MIC1/4/6 complex clustering host factors.

Perforation of the host cell plasma membrane during Toxoplasma gondii invasion requires rhoptry exocytosis

This study confirms that Toxoplasma gondii induces a transient increase in host cell plasma membrane conductance during invasion, suggesting a perforation mechanism for rhoptry protein delivery.

5' untranslated regions tune Toxoplasma translation

This study identifies how 5' UTR features and upstream AUGs regulate translation efficiency in Toxoplasma gondii using ribosome profiling and synthetic reporter assays.

Metabolic Adaptability and Nutrient Scavenging in Toxoplasma gondii: Insights from Ingestion Pathway-Deficient Mutants

This study investigates the role of the ingestion pathway in Toxoplasma gondii nutrient acquisition by analyzing mutants lacking key pathway components, revealing reliance on biosynthetic pathways and highlighting the pathway's role in nutrient scarcity.

Alternative start codon selection shapes mitochondrial function during evolution, homeostasis, and disease

The paper identifies differentially-localized protein isoforms generated by alternative start codon selection and shows their role in mitochondrial function, evolution, and disease.

TgATG9 is required for autophagosome biogenesis and maintenance of chronic infection in Toxoplasma gondii

This study identifies TgATG9 as essential for autophagosome formation and chronic Toxoplasma gondii infection.